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WebOne hundred μg of lysates were prepared as described in the “Ribosome Fractionation” section and diluted in equal volume of buffer containing 50 mM Tris–HCl (pH 7.5) and 2 mM DTT. ... 10% TCA insoluble material (0.1 mg Protein) was collected on 2.5 cm GFA filter. Following washing with 90% EtOH, the filters were air-dried and placed in ... stillman college interesting facts
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